By Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung
This handbook presents technical protocols for musculoskeletal examine on a translational foundation, i.e. a disease-orientated strategy. It deals suggestions on numerous laboratory options, together with cellphone tradition and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal versions, CT- and MRI-based densitometry and microarchitectural research, biomechanics and sensible research of orthopedic kinesiology, and so forth. The content material is easy and simple, with illustrations and step by step strategies as a simple experimental reference for body of workers in easy and scientific musculoskeletal learn and schooling. This booklet will supply a different multidisciplinary platform for numerous professions -- not just orthopedics, but additionally biomedical engineering and biomaterial sciences -- concerning either simple and scientific drugs.
Contents: mobile tradition and Molecular Biology; Histology and Histomorphometry; Microscopy and Bioimaging; Laboratory Animal versions; X-Ray- and MRI-based Densitometry; Biomechanics and movement research
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Extra info for A Practical Manual for Musculoskeletal Research
Once it touches the liquid on the array, release it quickly so that the sample pushes out air bubbles as it forms a monolayer against the microarray surface. Small air bubbles trapped under the cover slip exit after several minutes at 62°C. h The microarray should be transferred quickly from the cassette to the washing buffer. Leaving the microarray at room temperature will lead to elevated background fluorescence. Either a microarray wash station (TeleChem) or staining dishes (Wheaton) can be used for this washing step.
R. qxd 8/12/2008 12:21 PM Page xxxi List of Contributors Andrew D. Holmes Department of Health Technology & Informatics The Hong Kong Polytechnic University, Hong Kong SAR China Lin Huang Division of Plastic and Reconstructive Surgery Department of Surgery The Chinese University of Hong Kong Prince of Wales Hospital Hong Kong SAR China Chi-Cheung Hui Department of Rehabilitation Sciences The Hong Kong Polytechnic University, Hong Kong SAR China Vivian Wing-Yin Hung Musculoskeletal Research Laboratory Department of Orthopaedics & Traumatology The Chinese University of Hong Kong, Hong Kong SAR China Anthony E.
The signal intensity should be corrected for background, and control measures should be included to measure nonspecific hybridization and variable hybridization across arrays. The aim of data mining is to convert the hybridization signals into numbers which can be used to build a gene expression matrix. The interpretation of microarray experiments is carried out by grouping the data according to similar expression profiles. Clustering is a way of simplifying large data sets by partitioning similar data into specific groups.
A Practical Manual for Musculoskeletal Research by Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung